- 0
- By projectassemble
Recombinant Protein Expression Services
Recombinant Protein Expression Services
Protein Expression Services companies face a critical question when it comes to building or outsourcing key pre-clinical discovery capabilities. While establishing in-house capacities can be cost effective, it can also limit flexibility for fluctuating research and development demands. As a result, many organizations are turning to third-party providers for recombinant protein expression services to gain access to a wider range of scales and throughputs that can accelerate discovery workflows.
Recombinant protein production begins at the genetic level where a coding sequence for the desired protein is isolated and cloned into an expression vector. The vector often contains additional sequences (strong promoters, translation initiation signals, regulatory elements, resistance markers etc.) to increase the chances of a successful expression. The vector is then transformed into a host cell that is suitable for the target protein of interest. Once the protein is expressed, it is typically lysed and purified using techniques that are optimized for the specific construct design and protein characteristics (e.g., affinity chromatography for His-tagged proteins or GST chromatography for GST-tagged proteins).
Tailored Antibody Services for Research and Diagnostics
Depending on the desired protein characteristics and downstream applications, higher eukaryotic systems might be better suited to the task at hand. For instance, some proteins are insoluble when expressed in bacterial systems and may form insoluble aggregates that can be difficult to extract. In such cases, it might be beneficial to utilize baculovirus systems that provide excellent DNA replication capacity and accommodate large foreign DNA insert fragments, while still providing adequate solubility for recombinant protein production.
Read More